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Related Experiment Video

Updated: May 11, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Guidelines and considerations for conducting experiments using tissue microarrays.

Mohammad Ilyas1, Heike Grabsch, Ian O Ellis

  • 1School of Molecular Medical Sciences, Queen's Medical Centre, Nottingham University, Nottingham, UK.

Histopathology
|May 16, 2013
PubMed
Summary

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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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This summary is machine-generated.

Tissue microarrays (TMAs) are valuable for biomarker studies but require careful planning. This guide offers best practices for TMA experimental design, execution, and analysis to improve research reproducibility and biomarker discovery.

Area of Science:

  • Biomedical research
  • Pathology
  • Biomarker discovery

Background:

  • Tissue microarrays (TMAs) enable large-scale tissue-based biomarker studies.
  • Specific challenges exist in TMA methodology that require careful consideration.
  • Standardized quality control is crucial for robust and reproducible TMA research.

Framework:

  • Guidance for TMA experimental design, execution, and data analysis.
  • Recommendations derived from a National Cancer Research Institute workshop.
  • A checklist for planning and interpreting TMA experiments.

Implementation:

  • Covers pre-analytical (design), analytical (data acquisition), and post-analytical (data analysis) stages.
  • Addresses common pitfalls in TMA-based research.

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A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Related Experiment Videos

Last Updated: May 11, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

  • Aims to enhance the reliability of biomarker identification.

    • Implications:

    • Improves the robustness and reproducibility of TMA studies.
    • Facilitates the identification of clinically useful cancer biomarkers.
    • Complements existing guidelines like REMARK for cancer biomarker research.