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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Advances in superresolution optical fluctuation imaging (SOFI).

Thomas Dertinger1, Alessia Pallaoro, Gary Braun

  • 1Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA. t.dertinger@chem.ucla.edu

Quarterly Reviews of Biophysics
|May 16, 2013
PubMed
Summary
This summary is machine-generated.

Superresolution optical fluctuation imaging (SOFI) offers a powerful method for achieving higher resolution microscopy. This review covers SOFI

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Area of Science:

  • Optics and Photonics
  • Microscopy
  • Biophysics

Background:

  • Conventional optical microscopy is limited by diffraction, hindering detailed visualization of subcellular structures.
  • Superresolution microscopy techniques overcome diffraction limits, enabling nanoscale imaging.
  • Superresolution Optical Fluctuation Imaging (SOFI) is a computational approach to enhance image resolution.

Purpose of the Study:

  • To provide a comprehensive review of the Superresolution Optical Fluctuation Imaging (SOFI) technique.
  • To compare SOFI with other superresolution imaging methods, highlighting its unique attributes and trade-offs.
  • To explore the potential applications of SOFI in various imaging contexts.

Main Methods:

  • Review of SOFI principles and theoretical underpinnings.
  • Comparative analysis of SOFI performance against other superresolution techniques (e.g., STORM, PALM, STED).
  • Presentation of superresolved images generated using SOFI on diverse samples.

Main Results:

  • SOFI achieves superresolution by exploiting temporal fluctuations in fluorescence signals.
  • Demonstrated successful superresolution imaging of samples labeled with quantum dots, organic dyes, and plasmonic nanoparticles.
  • SOFI exhibits distinct advantages and limitations compared to other superresolution modalities.

Conclusions:

  • SOFI is a versatile superresolution technique with broad applicability.
  • Future prospects include live-cell imaging and application to non-fluorescent contrast mechanisms.
  • SOFI holds promise for advancing biological and materials science imaging.