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Updated: May 11, 2026
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Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
Published on: February 9, 2021
Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry
James J Miller1, Susanta K Hui, George S Jackson
1Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.
Summary
This study introduces a simplified method for calcium oxalate precipitation from large human urine volumes. The improved assay enhances calcium-41 detection for long-term biological monitoring.
Area of Science:
- Analytical Chemistry
- Biomedical Science
- Radiochemistry
Background:
- Calcium oxalate precipitation is crucial for preparing biological samples for calcium-41 (41Ca) analysis using accelerator mass spectrometry.
- Accurate long-term monitoring of 41Ca requires sensitive detection methods in human urine samples.
Purpose of the Study:
- To develop and characterize a simplified, large-volume protocol for calcium oxalate precipitation from human urine.
- To improve the signal-to-noise ratio for 41Ca:Ca measurements in biological samples.
Main Methods:
- Characterization of a simplified calcium oxalate precipitation protocol for large-volume human urine samples.
- Analysis of 41Ca:Ca ratios using accelerator mass spectrometry.
Main Results:
- Statistically significant increases in ion current were observed.
- Statistically significant decreases in interference were achieved.
- The large-volume assay demonstrated improved cost-effectiveness and reduced effort.
Conclusions:
- The simplified, large-volume calcium oxalate precipitation method enhances 41Ca analysis sensitivity.
- This protocol optimizes the time window for detecting 41Ca:Ca ratios significantly above background in lifetime human sample collections.
- The method is suitable for long-term biomedical monitoring and research involving 41Ca.
