Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the gene cluster for the dithiolopyrrolone antibiotic holomycin in Streptomyces clavuligerus.

Proceedings of the National Academy of Sciences of the United States of America·2010
Same author

Safety evaluation of tea (Camellia sinensis (L.) O. Kuntze) flower extract: assessment of mutagenicity, and acute and subchronic toxicity in rats.

Journal of ethnopharmacology·2010
Same author

Influences of soil properties and leaching on nickel toxicity to barley root elongation.

Ecotoxicology and environmental safety·2010
Same author

Effects of CO2 insufflation on cerebrum during endoscopic thyroidectomy in a porcine model.

Surgical endoscopy·2010
Same author

Plants' use of different nitrogen forms in response to crude oil contamination.

Environmental pollution (Barking, Essex : 1987)·2010
Same author

Overexpression of p35 in Min6 pancreatic beta cells induces a stressed neuron-like apoptosis.

Journal of the neurological sciences·2010

Related Experiment Video

Updated: May 11, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

The analysis of time-resolved optical waveguide absorption spectroscopy based on positive matrix factorization.

Ping Liu1, Zhu Li, Bo Li

  • 1Department of Automation, University of Science and Technology of China, Hefei 230026, China.

Journal of Colloid and Interface Science
|May 16, 2013
PubMed
Summary

Positive matrix factorization (PMF) effectively analyzes time-resolved optical waveguide absorption spectroscopy (OWAS) data for interfacial kinetics. This method provides dynamic, interpretable results for complex molecular interactions.

More Related Videos

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

Related Experiment Videos

Last Updated: May 11, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Time-resolved optical waveguide absorption spectroscopy (OWAS) utilizes evanescent fields to study polarized absorption spectra of sub-monomolecular adlayers.
  • OWAS is applicable to kinetics at solid/liquid interfaces involving diverse molecules like dyes, nanoparticles, and proteins.

Purpose of the Study:

  • To introduce and validate the application of Positive Matrix Factorization (PMF) for analyzing time-resolved OWAS data.
  • To compare the performance of PMF with Principal Component Analysis (PCA) for this analytical task.

Main Methods:

  • Time-resolved optical waveguide absorption spectroscopy (OWAS) was employed to collect spectral data.
  • Positive Matrix Factorization (PMF) and Principal Component Analysis (PCA) were utilized for data analysis.
  • Kinetics of Rhodamine 6G (R6G) adsorption/desorption and Meisenheimer complex formation were studied as model systems.

Main Results:

  • Time-resolved OWAS successfully recorded R6G adsorption/desorption kinetics and Meisenheimer complex formation dynamics.
  • PMF analysis yielded dynamic spectral features consistent with traditional time/wavelength-absorbance analysis.
  • PMF analysis avoided non-physical negative factors and produced more interpretable results compared to PCA.

Conclusions:

  • PMF is a valuable tool for analyzing complex time-resolved OWAS data, offering dynamic and interpretable insights.
  • The application of PMF enhances the study of interfacial kinetics and molecular dynamics.
  • PMF presents a promising analytical approach for increasingly large and complex spectroscopic datasets.