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Updated: May 11, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
Mechanism of one-electron oxidation of metformin in aqueous solution
P Trouillas1, C Marchetti, D Bonnefont-Rousselot
1INSERM UMR-S850, Faculté de Pharmacie, Université de Limoges, Limoges, France. patrick.trouillas@unilim.fr
Abstract:
Hydroxyl free radical-induced oxidation of metformin was studied in aqueous solution as a function of the pH. Hydroxyl free radicals were generated by gamma radiolysis of water and the oxidation end-products were quantified by high-performance liquid chromatography coupled to mass spectrometry (HPLC/MS), as a function of the radiation dose. This work is a joint experimental and theoretical (DFT) approach that has paved the way towards a comprehensive rationalization of the one-electron mechanisms of MTF oxidation, as a function of the pH.
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