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Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed

Rémi L Boulineau1, Mark A Osborne

  • 1Department of Chemistry, School of Life Sciences, University of Sussex, Falmer, BN1 9QJ, UK.

Chemical Communications (Cambridge, England)
|May 16, 2013
PubMed

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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.