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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

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Published on: May 5, 2016

Chemiluminescence detector based on a single planar transparent digital microfluidic device.

Xiangyu Zeng1, Kaidi Zhang, Jian Pan

  • 1Department of Microelectronics, Fudan University, 220 Handan RD, Shanghai 200433, China.

Lab on a Chip
|May 16, 2013
PubMed
Summary

A new portable chemiluminescence detector uses electrowetting-on-dielectrics (EWOD) for enhanced sensitivity. This compact device shows promise for sensitive, cost-effective immuno-detection, including blood glucose monitoring.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Chemiluminescence detection offers high sensitivity but often requires complex instrumentation.
  • Electrowetting-on-dielectrics (EWOD) provides a platform for manipulating droplets, enabling miniaturization of analytical devices.
  • Developing portable and cost-effective diagnostic tools is crucial for widespread healthcare applications.

Purpose of the Study:

  • To develop a compact and portable chemiluminescence detector prototype.
  • To investigate the use of a single polar transparent electrowetting-on-dielectrics (EWOD) device for enhanced detection.
  • To explore the relationship between EWOD contact angle and detection sensitivity.

Main Methods:

  • Fabrication of a single planar transparent EWOD device utilizing a coupling ground model for single polar voltage operation.
  • Optimization of the EWOD device by adjusting the contact angle to enhance fluorescence focusing.
  • Performance evaluation of the prototype detector using hydrogen peroxide (H2O2) as a model analyte.

Main Results:

  • The EWOD device design simplified chip construction and control circuits.
  • Increased contact angle on the EWOD surface correlated with stronger optical signals and higher sensitivity.
  • The prototype achieved a sensitivity of 5.45 mV (mmol L(-1))(-1) and a detection limit of 0.01 mmol L(-1) for H2O2 at a 120° contact angle.

Conclusions:

  • The compact EWOD-based chemiluminescence detector demonstrates high sensitivity and potential for miniaturization.
  • Further improvements in sensitivity and detection limits can be achieved by increasing the EWOD contact angle and reducing photomultiplier dark current.
  • The technology holds significant promise for cost-effective, portable immuno-detectors, particularly for applications like blood glucose monitoring.