Sensitive assay for carvedilol in tablets and spiked human plasma using a flow-injection chemiluminometric method

Area of Science:

• Analytical Chemistry
• Electrochemistry

Background:

• Carvedilol is an important cardiovascular drug.
• Accurate quantification of carvedilol is crucial for therapeutic monitoring and quality control.
• Existing analytical methods may lack sensitivity or require complex procedures.

Purpose of the Study:

• To develop a simple, sensitive, and rapid chemiluminometric method for carvedilol determination.
• To validate the method for analyzing carvedilol in pharmaceutical formulations and biological matrices.

Main Methods:

• Utilized flow injection (FI) analysis coupled with chemiluminescence (CL) detection.
• Employed the reaction of carvedilol with tris(2,2'-bipyridyl)ruthenium(II) and potassium permanganate (KMnO4) in a sulfuric acid medium.
• Optimized reaction conditions to maximize CL intensity and ensure linearity.

Main Results:

• Achieved a linear relationship between CL intensity and carvedilol concentration from 0.04-1.0 µg/mL.
• Established a low detection limit of 0.025 µg/mL (S/N=3).
• Demonstrated high precision with a relative standard deviation of 0.95% and a sample throughput of 90 samples/hour.

Conclusions:

• The developed FI-CL method offers a sensitive and efficient approach for carvedilol quantification.
• The method is successfully applied to the determination of carvedilol in tablets and spiked human plasma.
• This technique provides a valuable tool for routine analysis and quality control of carvedilol.