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Updated: May 11, 2026
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
A novel high performance liquid chromatographic method for simultaneous determination of ceftriaxone and sulbactam in
Sanjay Mohan Shrivastava1, Rajkumar Singh, Abu Tariq
1Venus Medicine Research Centre, Hill Top Industrial Estate, EPIP Phase I, Jharmajri (Extn.) Bhatoli Kalan, Baddi, Solan (H. P.), India.
Abstract:
An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide adjusted to pH7.0 with orthophosphoric acid in ratio 70:30. The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. Linearity, accuracy and precision were found to be acceptable over the concentration range of 125-750 ppm for ceftriaxone sodium and 62.5-375 ppm for sulbactam sodium. The LC method can be used for the quality control of formulated products containing ceftriaxone and sulbactam.
