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A Validated Method without Derivatization for the Determination of Gabapentin in Bulk, Pharmaceutical Formulation and
Rajinder Singh Gujral1, Sk Manirul Haque
1Vardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali, Punjab, India.
Abstract:
A rapid, sensitive and accurate high performance liquid chromatography with UV detection method was developed and validated for the quantification of gabapentin in bulk, pharmaceutical formulation and human urine samples. Most of the published methods for analysis of gabapentin used derivatization with reagent. The present paper however describes the analysis of gabapentin without any derivatization. The chromatographic separation was carried out on a Waters C18 5 μm column (150 mm × 4.6 mm) using a mixture of methanol - acetonitrile - potassium dihydrogen orthophosphate (pH5.2; 0.028 M) (25:10:65) as a mobile phase with UV detection at 210 nm. The method was linear over the range of 0.1-3.8 mg/ml of gabapentin. The within day and between day precision values are very good.
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