Related Experiment Video
Updated: May 11, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Development and Validation of Pregabalin in Bulk, Pharmaceutical Formulations and in Human Urine Samples by UV
Rajinder Singh Gujral1, Sk Manirul Haque, Prem Shanker
1Vardhman Chemtech Limited, Nimbua, Dera Bassi, Mohali, Punjab, India.
Abstract:
A simple and sensitive UV spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.5-5.0 μg/ml. There is no generally accepted method for the determination of pregabalin. The absorbance was measured at 210 nm. The method was validated with respect to accuracy, precision, specificity, ruggedness, and robustness, limit of detection and limit of quantitation. This method was used successfully for the quality assessment of five pregabalin drug products and in human urine samples with good precision and accuracy. This is found to be simple, specific, precise, accurate, reproducible and low cost UV Spectrophotometric method.
Related Concept Videos
Clinically Relevant Drug Product Specifications: Methods of Establishment
UV–Vis Spectrometers
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
Measurement of Bioavailability: Pharmacokinetic Methods
In Vitro Drug Release Testing: Overview, Development and Validation
