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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Published on: December 12, 2019
Notch signaling maintains neural rosette polarity
Heather Main1, Jelena Radenkovic, Shao-bo Jin
1Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden. h.main@physics.usyd.edu.au
Plos One
|May 16, 2013
Summary
Notch signaling is crucial for maintaining apical-basal cell polarity and neural stem cell populations. Loss of Notch signaling disrupts neural tube formation and rosette structure during nervous system development.
Area of Science:
- Developmental Biology
- Neuroscience
- Cell Biology
Background:
- Apical-basal cell polarity is fundamental for metazoan body plan formation and neural tube development.
- Proper cell polarity ensures neural stem cell maintenance and neural tube closure.
Purpose of the Study:
- To investigate the role of Notch signaling in apical-basal cell polarity during neural differentiation.
- To elucidate how Notch signaling influences neural tube development and stem cell behavior.
Main Methods:
- Utilized CSL(-/-) mouse embryos and embryonic stem (ES) cells lacking canonical Notch signaling.
- Employed pharmacological inhibition of Notch signaling, actin polymerization, and Rho kinase (ROCK) activity.
- Observed neural tube defects, rosette formation, and neuronal differentiation markers.
Main Results:
- CSL(-/-) embryos exhibited neural tube defects, indicating cell polarity and convergent extension issues.
- Notch signaling (CSL) is required for correct rosette structure maintenance and neuronal differentiation regulation in vitro.
- While actin and ROCK activity are vital for rosette integrity, their disruption did not impede neuronal differentiation.
Conclusions:
- Notch signaling is essential for maintaining apical-basal polarity and organization in the developing nervous system.
- The study highlights Notch signaling's dual role in both differentiation and structural organization.
- Rosette maintenance, though dependent on Notch, actin, and ROCK, is not a prerequisite for neuronal differentiation.
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
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