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Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
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Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

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Updated: May 11, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Published on: June 6, 2017

Lactobacillus decelerates cervical epithelial cell cycle progression.

Katarina Vielfort1, Linda Weyler, Niklas Söderholm

  • 1Department of Molecular Biosciences, The Wenner-Grens Institute, Stockholm University, Stockholm, Sweden.

Plos One
|May 16, 2013
PubMed
Summary

Certain Lactobacillus species, like L. rhamnosus and L. reuteri, inhibit cervical cancer cell proliferation by halting cell cycle progression. L. crispatus, however, shows no such effect.

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Published on: May 14, 2016

Area of Science:

  • Microbiology
  • Cell Biology
  • Cancer Research

Background:

  • Lactobacillus species are known for their diverse roles in human health.
  • Understanding their interaction with host cells, particularly cancer cells, is crucial for potential therapeutic applications.

Purpose of the Study:

  • To investigate the impact of different Lactobacillus species on the cell cycle progression of human cervical carcinoma ME-180 cells.
  • To elucidate the mechanisms by which Lactobacillus species influence cancer cell proliferation.

Main Methods:

  • Live cell microscopy
  • Bromodeoxyuridine incorporation assays
  • Flow cytometry analysis of cell cycle phases
  • Analysis of p21 protein expression and localization

Main Results:

  • L. rhamnosus and L. reuteri colonization reduced ME-180 cell cycle progression, causing G1 phase arrest.
  • L. rhamnosus-induced G1 arrest was associated with increased p21 expression and nuclear accumulation.
  • L. crispatus did not significantly alter ME-180 cell cycle progression.
  • Supernatants from L. rhamnosus and lactic acid itself inhibited ME-180 cell proliferation.

Conclusions:

  • Lactobacillus species exhibit diverse effects on cervical cancer cell cycle progression.
  • L. rhamnosus and L. reuteri possess anti-proliferative properties against human cervical carcinoma cells, potentially mediated by lactic acid and p21.
  • These findings highlight the potential of specific Lactobacillus strains in cancer therapy.