Collagen loaded nano-sized surfactant based dispersion for topical application: formulation development,...

Area of Science:

• Dermatology
• Nanotechnology
• Cosmetic Science

Background:

• Collagen possesses significant anti-aging, anti-wrinkle, and wound healing properties.
• The high molecular weight and hydrophilic nature of collagen impede effective topical delivery.
• Developing advanced delivery systems is crucial for enhancing collagen's therapeutic potential.

Purpose of the Study:

• To formulate and characterize an effective topical nano-surfactant dispersion (NSD) for improved collagen delivery.
• To evaluate the skin hydration, safety, and efficacy of the developed collagen-loaded NSD.
• To overcome the limitations of conventional topical collagen formulations.

Main Methods:

• Nano-surfactant dispersions (NSD) were prepared using sorbitan monostearate (Span60) and cholesterol via the ethanol injection method followed by probe sonication.
• Characterization included entrapment efficiency (%EE), particle size (z-avg), polydispersity index (PDI), zeta-potential (ζ), in vitro release, skin hydration, and skin irritation tests.
• Histopathological examinations were conducted to assess skin safety.

Main Results:

• The optimized NSD (NSD3) exhibited favorable characteristics: %EE (77.56%), z-avg (158.1 nm), PDI (0.211), and ζ-potential (-17.2 mV).
• In vivo studies showed NSD treatment increased stratum corneum thickness by 2.5-3 fold compared to controls.
• Skin irritation tests in rats and rabbits indicated a non-irritant nature, and histopathology revealed no significant adverse skin changes.

Conclusions:

• The developed nano-surfactant dispersion provides an effective topical delivery system for collagen.
• The NSD formulation significantly enhances skin hydration and demonstrates a favorable safety profile.
• This nanotechnology-based approach offers a promising solution for topical collagen application in cosmetic and therapeutic settings.