Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between preoperative prognostic nutritional index and postoperative delirium in older adults with hip fractures: a real-world study.

Annals of medicine·2026
Same author

Vibrio coralliilyticus: A temperature-driven coral pathogen-Genomic virulence, environmental interactions, and integrated biocontrol strategies.

Marine pollution bulletin·2026
Same author

Effect of controlled hypotension combined with low-pressure tourniquet on hemoglobin reduction after total knee arthroplasty.

BMC surgery·2026
Same author

Corrigendum to "Sodium cyclamate enhances Vibrio parahaemolyticus biofilm formation on seafood-contact surfaces" [Food Res. Int. 235 (2026) 119195].

Food research international (Ottawa, Ont.)·2026
Same author

Sodium cyclamate enhances Vibrio parahaemolyticus biofilm formation on seafood-contact surfaces.

Food research international (Ottawa, Ont.)·2026
Same author

Antimicrobial and anti-biofilm activity of Epigallocatechin gallate against Vibrio parahaemolyticus.

NPJ science of food·2026

Related Experiment Video

Updated: May 11, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Bcl10 is an essential regulator for A20 gene expression.

Wu Xu1, Liquan Xue, Yi Sun

  • 1Department of Chemistry, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA, wxx6941@louisiana.edu.

Journal of Physiology and Biochemistry
|May 17, 2013
PubMed
Summary

Bcl10 enhances A20 gene expression in B lymphocytes, impacting B-cell proliferation. This study reveals Bcl10 as a key regulator in B-cell receptor signaling pathways.

More Related Videos

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Related Experiment Videos

Last Updated: May 11, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • A20 is a tumor suppressor involved in lymphomas and regulates nuclear factor kappa B (NF-κB).
  • Bcl10 activation is linked to mucosa-associated lymphoid tissue lymphomas and is crucial for NF-κB activation in B cells.

Purpose of the Study:

  • To investigate the regulatory role of Bcl10 in A20 gene expression.
  • To explore the impact of Bcl10 on B-cell proliferation.
  • To analyze the structural properties of Bcl10.

Main Methods:

  • Investigated A20 gene expression in B lymphocytes using B-cell receptor engagement.
  • Performed transient transfection assays in HEK 293 cells to assess A20 promoter activity.
  • Utilized molecular modeling and dynamics simulation to analyze Bcl10 structure.
  • Examined B-cell proliferation in BCL10-transgenic mice.

Main Results:

  • Bcl10 upregulates endogenous A20 gene expression in B lymphocytes.
  • Bcl10 activates the A20 promoter, which contains NF-κB-binding sites.
  • Marginal zone B cells from BCL10-transgenic mice show increased proliferation compared to wild-type cells.

Conclusions:

  • Bcl10 is an essential regulator of A20 gene expression.
  • Bcl10 plays a significant role in B-cell proliferation mediated by B-cell receptor signaling.