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Updated: May 11, 2026

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Published on: January 9, 2020
Promoters of cancer genes for recombinant protein expression in human cancer cell lines
Mohammad Pourhassan-Moghaddam1, Behrouz Farhadi, Kazem Nejati-Koshki
1Department of Medical Biotechnology, School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran ; Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran ; Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Introduction:
Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges.
Methods:
In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors.
Results:
After designing, these vectors could be applied to produce complex hu-man recombinant proteins in the human cancer cell lines as production hosts.
Conclusion:
Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
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