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Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: May 11, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

Recombinant proteins: hopes for tissue engineering.

Mohammad Morad Farajollahi¹, Sepideh Hamzehlou, Ahmad Mehdipour

¹Department of Medical Biotechnology, Cellular and Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Bioimpacts : BI

|May 17, 2013

Summary

This summary is machine-generated.

Recombinant proteins offer significant advantages over natural proteins for tissue engineering applications. This technology allows for the design of improved proteins, benefiting both medical and economic aspects of tissue engineering programs.

Keywords:

Gene Therapy Recombinant Proteins Tissue Engineering

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Area of Science:

Biomaterials Science
Regenerative Medicine
Molecular Biology

Background:

Proteins are crucial molecules in tissue engineering.
Natural proteins have limitations for engineered applications.
Recombinant proteins offer a viable alternative.

Purpose of the Study:

To review the development and applications of recombinant proteins in tissue engineering.
To highlight the advantages of recombinant proteins over natural sources.
To emphasize the potential of recombinant proteins in advancing tissue engineering.

Main Methods:

Development of recombinant forms of structural proteins, growth factors, and cytokines.
Engineering of proteins with specific functionalities, such as matrix-binding domains.
Production of recombinant proteins via ex vivo methods and in vivo gene therapy.

Main Results:

Successful development of various recombinant proteins for tissue engineering.
Creation of engineered proteins with enhanced properties (e.g., improved mechanical strength, targeted binding).
Demonstration of medical and economic benefits of recombinant protein production.

Conclusions:

Recombinant protein technology overcomes limitations of natural proteins in tissue engineering.
Engineered proteins offer tailored solutions for tissue regeneration.
Recombinant proteins should be integrated into all tissue engineering programs for optimal outcomes.