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Updated: May 11, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
[Selection of reference genes for virulence gene expression in Vibrio parahaemolyticus]
Qin Li1, Zhiyun Peng, Xinpeng Chen
1College of Food Science and Technology, Shanghai Ocean University, Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China. lqapple1988@yahoo.com.cn
Objective:
We selected reference genes for virulence gene expression of Vibrio parahaemolyticus under different environmental conditions.
Method:
Using qRT-PCR, we evaluated the expression stability of four housekeeping genes (GAPDH, pvuA, pvsA and rpoS) of V. parahaemolyticus cultivated separately in seawater, filtered seawater, shrimp and Tryptone Soya Broth.
Result:
The result shows that all the candidate reference genes could be amplified specifically in qRT-PCR reaction. The expression stability of the four reference genes ranked pvuA (2.906) > pvsA (3.197) > GAPDH (3.746) > rpoS (6.512). Further analysis with geNorm program reveals that the highest stability was observed in pvuA and pvsA. The geometric average score of the two genes was considered as the most appropriate reference gene for normalizing the expression of virulence genes of V. parahaemolyticus.
Conclusion:
The pvuA and pvsA genes could be used as reference genes to study the virulence gene expression of V. parahaemolyticus.
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