[Effect of autoinducer 2 on Riemerell antatipestifer adherence and invasion to Vero cells]
Related Experiment Video
Updated: May 11, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Published on: May 16, 2011
Lei Liu1, Xiangan Han, Rui Liu
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China. liulei0606@gmail.com
Objective:
Autoinducer 2 (AI-2), used to communicate among bacterial species, regulates numerous physiological functions of bacteria. In this study, we studied the effect of AI-2 on adherence and invasion of Riemerella antatipestifer (RA) strain CH3 to Vero cells and transcriptional levels of virulence-related and metabolism-related genes were investigated.
Methods:
To verify whether the adherence and invasion of CH3 was affected by AI-2, we added different concentrations of AI-2 to the cocultures of Vero cells and CH3 and then calculated adherence percentages and invasion percentages of tested groups. We further added AI-2 (184.0 micromol/L) to the tryptone soya broth culture of CH3 and then detected the effect of transcriptional levels of related genes of CH3 using real-time PCR.
Results:
The adherence of CH3 to Vero cells was decreased most to 62% with 18.4 micromol/L AI-2 and the invasion of CH3 to Vero cells was increased most to 194% with 184.0 micromol/L AI-2. The result of real-time PCR shows that AI-2 increased transcriptional levels of some virulence-related genes and decreased transcriptional levels of some metabolism-related genes.
Conclusion:
These results suggest that AI-2 affected adherence and invasion of CH3 to Vero cells. Moreover, AI-2 could regulate some genes of CH3 to modulate particular physiological behaviors.
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Regulation of Bacterial Virulence
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Intracellular Movement of Viruses and Bacteria

