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Published on: February 2, 2018
Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a
Raúl Teruel1, Irene Martínez-Martínez, José A Guerrero
1Centro Regional de Hemodonación, University of Murcia, IMIB, Spain, C/Ronda de Garay S/N, 30003, Murcia, Spain.
Background:
Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant.
Results:
In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control.
Conclusions:
These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.
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