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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Spectroscopy of Carboxylic Acid Derivatives01:26

Spectroscopy of Carboxylic Acid Derivatives

Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and unsymmetrical carbonyl vibration.
In the...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

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Automated Analysis of Dynamic Ca2+ Signals in Image Sequences
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CAPITO--a web server-based analysis and plotting tool for circular dichroism data.

Christoph Wiedemann1, Peter Bellstedt, Matthias Görlach

  • 1Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research-Fritz Lipmann Institute, Beutenbergstr. 11, 07745 Jena, Germany. cwiede@fli-leibniz.de

Bioinformatics (Oxford, England)
|May 18, 2013
PubMed
Summary

A new web tool, CAPITO (CD Analysis and Plotting Tool), simplifies the analysis and visualization of circular dichroism (CD) spectroscopy data for protein folding studies. It offers reliable secondary structure estimation and handles multiple datasets efficiently.

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Bioinformatics

Background:

  • Circular dichroism (CD) spectroscopy is crucial for studying protein folding and verifying protein structure.
  • Analyzing multiple CD datasets often requires specialized tools and expertise.
  • Existing methods can be format-dependent and lack user-friendliness.

Purpose of the Study:

  • To develop a user-friendly, platform-independent tool for analyzing circular dichroism data.
  • To provide high-quality graphical output for CD spectroscopy results.
  • To facilitate the analysis of protein folding dynamics and structural comparisons.

Main Methods:

  • Development of a novel web server-based tool named CAPITO (CD Analysis and Plotting Tool).
  • Implementation of algorithms for reliable estimation of protein secondary structure content.
  • Support for analyzing multiple CD datasets in various formats.

Main Results:

  • CAPITO enables efficient analysis and plotting of CD data.
  • The tool provides reliable secondary structure content estimation using multiple approaches.
  • It is suitable for analyzing temperature/pH-dependent unfolding and comparing protein mutants.

Conclusions:

  • CAPITO offers an accessible solution for CD data analysis in protein research.
  • The tool enhances the study of protein folding and structural integrity.
  • It supports a wide range of applications in structural biology and biochemistry.