Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biofabrication of an ovine intervertebral disc model by combining a polycaprolactone frame with a bioprinted alginate hydrogel.

Biofabrication·2025
Same author

Modulation of SARS-CoV-2 spike binding to ACE2 through conformational selection.

Nature nanotechnology·2025
Same author

Spot Spine, a freely available ImageJ plugin for 3D detection and morphological analysis of dendritic spines.

F1000Research·2024
Same author

Orphan nuclear receptors-induced ALT-associated PML bodies are targets for ALT inhibition.

Nucleic acids research·2024
Same author

Real-time monitoring of replication errors' fate reveals the origin and dynamics of spontaneous mutations.

Nature communications·2024
Same author

Transient demyelination causes long-term cognitive impairment, myelin alteration and network synchrony defects.

Glia·2024

Related Experiment Video

Updated: May 11, 2026

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
09:39

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

Published on: December 16, 2022

TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization.

Jean Ollion1, Julien Cochennec, François Loll

  • 1CNRS UMR7196, INSERM U565, MNHN, 43 rue Cuvier 75005 Paris, France.

Bioinformatics (Oxford, England)
|May 18, 2013
PubMed
Summary

Tools for Analysis of Nuclear Genome Organization (TANGO) is a new software enabling biologists to analyze 3D nuclear images. This tool simplifies complex image processing and statistical analysis for better understanding of nuclear organization.

More Related Videos

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types
06:33

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types

Published on: June 28, 2024

Related Experiment Videos

Last Updated: May 11, 2026

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis
09:39

TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis

Published on: December 16, 2022

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types
06:33

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types

Published on: June 28, 2024

Area of Science:

  • Cellular Biology
  • Biophysics
  • Genomics

Background:

  • The cell nucleus, containing genetic material, is a highly organized organelle.
  • Understanding nuclear architecture is crucial for cellular biology.
  • Quantitative analysis of 3D fluorescence images aids in studying nuclear compartment functions but is limited by processing demands.

Purpose of the Study:

  • To introduce Tools for Analysis of Nuclear Genome Organization (TANGO), an image analysis tool for nuclear architecture studies.
  • To provide a comprehensive framework for the complete analysis of 3D fluorescence images of the cell nucleus.
  • To enable biologists to perform quantitative studies of nuclear organization without extensive programming skills.

Main Methods:

  • TANGO integrates ImageJ for image processing and quantitative analysis with R for statistical analysis.
  • It features an intuitive user interface for segmentation and analysis setup.
  • The tool is designed to handle large datasets of 3D fluorescence images.

Main Results:

  • TANGO offers a coherent framework for the entire 3D fluorescence image analysis workflow.
  • It allows precise segmentation and analysis setup through a user-friendly interface.
  • The tool facilitates the quantitative study of nuclear organization by processing large image sets.

Conclusions:

  • TANGO is a versatile, free, and open-source tool for the quantitative analysis of nuclear architecture.
  • It empowers biologists to conduct in-depth studies of nuclear organization using 3D fluorescence imaging.
  • The software is available for multiple operating systems and distributed under GPL v.2 license.