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Related Experiment Video

Updated: May 11, 2026

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

Texas 3-step decellularization protocol: looking at the cardiac extracellular matrix.

Lisandra E de Castro Brás1, Trevi A Ramirez, Kristine Y DeLeon-Pennell

  • 1San Antonio Cardiovascular Proteomics Center, USA. ldecastrobras@umc.edu

Journal of Proteomics
|May 18, 2013
PubMed
Summary

This study presents a novel method to solubilize extracellular matrix (ECM) proteins, overcoming challenges in proteomics. The optimized protocol enriches ECM components for accurate identification and quantification in tissue samples.

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Last Updated: May 11, 2026

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08:41

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Published on: December 4, 2017

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
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Published on: December 6, 2012

Area of Science:

  • Biochemistry
  • Proteomics
  • Tissue Engineering

Background:

  • Extracellular matrix (ECM) proteins provide structural and regulatory support but are difficult to study due to low solubility.
  • ECM proteins are often undetected in proteomics due to solubility issues and low abundance compared to other cellular proteins.

Purpose of the Study:

  • To develop and optimize a strategy for the enrichment and solubilization of ECM components for proteomic analysis.
  • To enable deeper investigation of ECM proteins in various biological and pathological conditions.

Main Methods:

  • Tissue decellularization followed by acid extraction and enzymatic deglycosylation to enhance ECM solubilization.
  • Generation of three distinct fractions: soluble, cellular, and insoluble ECM components.
  • Buffers compatible with mass spectrometry-based proteomics analysis.

Main Results:

  • A novel, optimized protocol for ECM enrichment and solubilization was successfully developed.
  • The protocol generates three fractions amenable to mass spectrometry analysis.
  • This method allows for the identification and quantification of ECM components, including those masked in standard analyses.

Conclusions:

  • The developed method effectively addresses the low solubility challenge of ECM proteins.
  • The three-fraction approach enables detection of ECM protein differences masked in global proteomic analyses.
  • This optimized protocol has immediate practical relevance for proteomics and immunoblotting of ECM components.