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Updated: May 11, 2026
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Published on: September 15, 2014
Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating
Asuka Morizane1, Daisuke Doi, Jun Takahashi
1Department of Clinical Application, Center for iPS Cell, Kyoto University, Kyoto, Japan.
Abstract:
Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter describes the feeder-free floating aggregation culture system for the induction of dopaminergic neurons. This method is simple and highly efficient for the production of dopaminergic neurons. It has several advantages for application in clinical usage in comparison to the other protocols using either feeder cells or Matrigel.
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