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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: May 11, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Cryosectioning.

Alexander I Son¹, Katie Sokolowski, Renping Zhou

¹Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 18, 2013

Summary

This summary is machine-generated.

Cryosectioning, a vital histological technique, involves sectioning frozen specimens. This guide details an effective method for embedding, mounting, and sectioning frozen tissues to achieve high-quality results.

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Last Updated: May 11, 2026

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Published on: December 12, 2016

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Published on: July 12, 2015

Area of Science:

Histology
Cryotechnology
Biotechnology

Background:

Cryosectioning is a long-established histological method.
Producing high-quality frozen sections requires significant expertise and patience.

Purpose of the Study:

To present an effective protocol for cryosectioning.
To offer guidance on achieving superior frozen tissue sections.

Main Methods:

Detailed method for embedding frozen tissues.
Step-by-step protocol for mounting frozen specimens.
Techniques for sectioning frozen tissues.

Main Results:

An effective cryosectioning method is described.
Suggestions for optimizing section quality are provided.

Conclusions:

This chapter offers a practical approach to cryosectioning.
The provided method aims to improve the success rate of obtaining high-quality frozen sections.