Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Transport Across the Golgi01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
COP Coated Vesicles00:59

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The SEQUINS scholars program: a longitudinal curriculum to advance brain health equity education among early career trainees.

Equity neuroscience·2026
Same author

AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1<sup>G93A</sup> ALS mice.

Gene therapy·2025
Same author

AdaGen: Learning Adaptive Policy for Image Synthesis.

IEEE transactions on pattern analysis and machine intelligence·2025
Same author

A Spontaneous Melanoma Mouse Model Applicable for a Longitudinal Chemotherapy and Immunotherapy Study.

The Journal of investigative dermatology·2023
Same author

Inhibitors of Keap1-Nrf2 protein-protein interaction reduce estrogen responsive gene expression and oxidative stress in estrogen receptor-positive breast cancer.

Toxicology and applied pharmacology·2023
Same author

Inducible motor neuron differentiation of human induced pluripotent stem cells in vivo.

Cell proliferation·2022

Related Experiment Video

Updated: May 11, 2026

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
09:44

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Published on: June 22, 2017

The Golgi-Cox method.

Gitanjali Das1, Kenneth Reuhl, Renping Zhou

  • 1Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 18, 2013
PubMed
Summary
This summary is machine-generated.

Golgi staining offers unparalleled clarity for visualizing brain cytoarchitecture and neuron morphology. This method, detailed in a standardized Golgi-Cox protocol, aids in studying genetic effects on neuronal structure and function.

More Related Videos

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
10:26

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Published on: April 18, 2017

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Related Experiment Videos

Last Updated: May 11, 2026

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
09:44

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Published on: June 22, 2017

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
10:26

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Published on: April 18, 2017

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Area of Science:

  • Neuroscience
  • Neuroanatomy
  • Cell Biology

Background:

  • Golgi staining is a key neurohistologic technique for visualizing neuronal morphology.
  • It relies on metallic impregnation to reveal entire neurons, including axons, dendrites, and spines.
  • Understanding neuronal structure is crucial for elucidating brain function.

Purpose of the Study:

  • To describe a standardized Golgi-Cox protocol for neurohistologic analysis.
  • To enable the study of genetic manipulation effects on neuronal structure.
  • To elucidate gene functions in brain development and adult function.

Main Methods:

  • Standardization of the Golgi-Cox staining protocol in a laboratory setting.
  • Application of the protocol to analyze experimental effects of genetic manipulations.
  • Microscopic examination of neuronal cytoarchitecture, dendrite density, and spine morphology.

Main Results:

  • The Golgi-Cox protocol provides high clarity for visualizing neuronal entirety.
  • The method allows for detailed analysis of spatial distribution and morphology of neurons.
  • It is effective in studying changes induced by genetic manipulations.

Conclusions:

  • The standardized Golgi-Cox protocol is a valuable tool for neurohistologic research.
  • This method facilitates the investigation of gene function in neuronal development and adult brain.
  • It offers a clear visualization of neuronal structures essential for understanding brain cytoarchitecture.