[Isolation and partial characteristics of biopolymers in the outer membrane of Pseudomonas syringae]

Conditions are developed for obtaining extracellular biopolymers (EBP), water (W), salt-EDTA (SE) and salt-detergent (SD) extracts of Pseudomonas syringae pv. atrofaciens IMV K-1025. The fraction of unsoluble LPS is effectively precipitated from samples by ultracentrifugation (UC). The fractions enriched by proteins and soluble LPS are salted-out from the UC supernatants by ammonium sulphate, that is confirmed by the chemical analysis, electrophoresis in the PAAG-NDS and in ELISA with the monoclonal antibodies against the homologous LPS, a comparative analysis of electrophoretic spectra of UC supernatants of EBP, W and SE extracts has shown their great similarity in the composition rather than in the specific content of basic and minor polypeptides. Nine polypeptides of the major membrane of Pseudomonas syringae pv. atrofaciens IMV K-1025 having the molecular weights of 15.5, 30.0, 34.0, 36.0, 38.0, 41.0, 42.0, 43.0 and 65.0 kDa are identified as major ones.