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[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27]

A D Iaremchuk, M A Tukalo, S P Egorova

Ukrainskii Biokhimicheskii Zhurnal (1978)

|March 1, 1990

Abstract:

A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.

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