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Updated: May 11, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection
1Institute of Environmental Engineering, Department of Physics, Dalian Maritime University, 1 Linghai Road, Dalian 116026, China. kyliu@cas.dicp.ac.cn
Abstract:
Capillary array electrophoresis (CAE) is a promising technique for multiple enantiomeric separations. Carboxytetramethylrhodamine succinimidyl ester (TAMRA SE), a rhodamine-core fluorescent probe, has rarely been applied as an original precolumn derivatization reagent for chiral amino acid (AA) analysis so far. For these purposes, high-throughput enantiomeric separations of 12 TAMRA SE-AAs by a home-made 532 nm CAE-LIF scanner are presented. The effect of cyclodextrins (CDs) and a variety of organic modifiers was quickly investigated. Baseline separations were achieved in 100 mM Tris-borate buffer (pH 10.0) containing 2 mM β-CD and 10 mM hexamethylenediamine (HDA). Multiple determination of the enantiomeric excess (ee) in non-racemic mixtures of alanine is successfully presented.
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