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"Prorenin" in human plasma?

J E Sealey, J H Laragh

    Circulation Research
    |June 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Plasma renin activity increases upon prolonged storage due to inactive renin activation, not changes in renin substrate or other factors. Low-renin hypertension patients show abnormal prorenin secretion during sodium depletion.

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    Area of Science:

    • Biochemistry
    • Endocrinology
    • Hypertension Research

    Background:

    • Plasma renin activity (PRA) is a key regulator of blood pressure.
    • Understanding factors affecting PRA measurement is crucial for accurate diagnosis and treatment of hypertension.
    • An inactive precursor to renin, termed 'prorenin', has been identified.

    Purpose of the Study:

    • To investigate the cause of increased plasma renin activity observed after prolonged storage of plasma.
    • To determine if storage affects renin substrate concentration or other plasma components influencing renin activity.
    • To explore the role of prorenin activation in normal subjects and patients with essential hypertension.

    Main Methods:

    • Plasma samples from normal subjects and patients with essential hypertension were stored at -20°C for prolonged periods.

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  • Plasma renin activity, renin substrate concentration, and angiotensin generation rates were measured.
  • Prorenin levels were assessed in response to sodium depletion and repletion in patients with low-renin hypertension.

    • Main Results:

    • Prolonged storage of plasma at -20°C led to an increase in plasma renin activity in normal subjects and most hypertensive patients.
    • Renin substrate concentration and angiotensin generation rates remained unchanged after storage, suggesting storage does not alter these components.
    • The observed increase in renin activity is consistent with the activation of an inactive renin form (prorenin).
    • Low-renin hypertension patients exhibited abnormal prorenin secretion during sodium depletion, which normalized upon sodium administration.

    Conclusions:

    • The increase in plasma renin activity after prolonged storage is likely due to the activation of prorenin.
    • This finding suggests that careful consideration of sample handling and storage is necessary for accurate renin activity measurements.
    • Abnormal prorenin secretion in response to sodium depletion may be a characteristic of certain low-renin hypertension subtypes.