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Published on: January 28, 2019
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients
Luciane A Pereira1, Carla E O Ferreira, Giovana D Turchetto
1Laboratório de Virologia, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
Insights
Group A rotavirus (RVA) infections in children can cause severe illness. Monitoring RVA
Area of Science:
- Virology
- Pediatrics
- Infectious Diseases
Background:
- Group A rotavirus (RVA) is a leading cause of severe gastroenteritis in infants and young children globally.
- Understanding the genotypic diversity of RVA is essential for public health surveillance and vaccine effectiveness evaluation.
Purpose of the Study:
- To characterize the genotypic variability of group A rotavirus (RVA) in pediatric patients.
- To investigate potential correlations between RVA genotypes and clinical outcomes, including severity of illness and nosocomial infections.
Main Methods:
- A cross-sectional study analyzed 1,140 stool samples from pediatric patients with acute gastroenteritis.
- Rotavirus diagnosis was confirmed using latex agglutination and enzyme immunoassay.
- Genotyping was performed using reverse transcription, multiplex hemi-nested polymerase chain reaction (PCR), and nucleotide sequencing.
Main Results:
- Eighty RVA-positive samples revealed common G-P genotype combinations: G4P[8] (38.9%), G1P[8] (30.5%), G9P[8] (13.9%), and G2P[4] (6.9%).
- The G2P[4] genotype became more prevalent post-vaccine implementation (2006-2008).
- Infants under 12 months were most affected, with high rates of severe dehydration and intensive care needs; 12.5% of infections were nosocomial. No genotype-severity correlation was found.
Conclusions:
- RVA infections in pediatric patients can lead to severe clinical manifestations.
- Continuous surveillance of RVA genotypic variability is critical for tracking emerging strains and assessing immunization program impact.
Objective:
To describe the genotypic variability of group A rotavirus (RVA) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the Hospital de Clínicas da Universidade Federal do Paraná (HC-UFPR), Curitiba, Paraná.
Methods:
A cross-sectional study was conducted with 1,140 stool samples collected from April, 2001 to December, 2008 in outpatients and hospitalized patients with acute gastroenteritis referred to the hospital. RVA diagnosis was performed through the latex agglutination method and enzyme immunoassay. Reverse transcription followed by multiplex hemi-nested polymerase chain reaction (PCR) and nucleotide sequencing were used for genotype characterization. Genotype combinations, clinical, epidemiological, laboratory data, and presence of hospital-acquired infections were reported.
Results:
A total of 80 rotavirus-positive stool samples were analyzed. The most frequent associations between genotypes G and P were: G4 P[8] (38.9%), G1 P[8] (30.5%), G9 P[8] (13.9%), G2 P[4] (6.9%), and G3 P[8] (1.4%). G2 P[4] was the most prevalent genotype after the vaccine implementation in the years 2006 and 2008. A total of 62,5% of infected children were aged less than 12 months. Of these, 55.6% had severe dehydration and 26.7% needed intensive care. A frequency of 12.5% of nosocomial infections was found. No correlation was observed between genotype and severity of infection in the study patients.
Conclusion:
RVA infections can be associated with severe clinical manifestations, and the surveillance of genotypic variability of this virus is crucial to monitor the emergence of new strains and the impact of the immunization in these patients.
