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Updated: May 11, 2026
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
Profiling celiac disease antibody repertoire.
Sara D'Angelo1, Flavio Mignone, Cecilia Deantonio
1Department of Health Sciences and Interdisciplinary Research Center on Autoimmune Diseases (IRCAD), University of Eastern Piedmont Amedeo Avogadro, Novara, Italy.
Clinical Immunology (Orlando, Fla.)
|May 21, 2013
Summary
Researchers identified thirteen celiac disease (CD)-specific antigens by analyzing autoantibody responses. This antibody pattern is gluten-independent and distinct from other autoimmune diseases, offering new diagnostic and research avenues.
Area of Science:
- Immunology
- Autoimmunity
- Gastroenterology
Background:
- Celiac disease (CD) diagnosis relies on identifying specific autoantibodies.
- The full spectrum of autoantibody targets in CD remains incompletely understood.
- Identifying disease-specific autoantigens is crucial for understanding CD pathogenesis.
Purpose of the Study:
- To characterize the autoantibody response in celiac disease.
- To identify disease-specific autoantigenic protein patterns (epitopes).
- To explore potential new diagnostic markers and research targets for autoimmunity.
Main Methods:
- Utilized a cDNA phage-display library to screen sera from CD patients for immunoreactive antigens.
- Identified candidate genes, produced corresponding proteins, and validated immunoreactivity.
- Confirmed antigen specificity using protein microarrays and ELISA, and tested against control sera and other autoimmune diseases.
Main Results:
- Identified thirteen novel CD-specific antigens.
- Validated the specificity of six antigens via ELISA.
- Demonstrated that the antibody response is gluten-independent and not shared with other autoimmune conditions.
Conclusions:
- The identified antigens represent a CD-specific antibody profile.
- This response is independent of gluten intake and distinct from other autoimmune diseases.
- These findings have implications for CD diagnostics and may reveal new targets for autoimmunity research.
