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A squaraine-based red emission off-on chemosensor for biothiols and its application in living cells imaging
Xiao-Dong Liu1, Ru Sun, Jian-Feng Ge
1Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Material Science, Soochow University, 199 Ren'Ai Road, Suzhou 215123, China.
Abstract:
A new probe based on the squaraine skeleton with a 2,4-dinitrobenzenesulfonyl unit is reported as a fluorescent probe for biothiol. It shows turn-on properties with red emission towards biothiols in acetonitrile-PBS buffer (1 : 9, v/v) solution, and its properties of rapid response, high selectivity and high sensitivity make it a potential probe with real applications. Moreover, the in vitro assays show that the probe can be used in fluorescence imaging for the detection of intracellular biothiol levels.
