Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
The Nucleus01:25

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
The Nucleus01:32

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HMGB1 regulates mitochondrial structure and reactive oxygen species balance during the transition from naïve to primed pluripotency.

Frontiers in cell and developmental biology·2026
Same author

Female Aging Affects Coilin Pattern in Mouse Cumulus Cells.

Journal of developmental biology·2026
Same author

Non-Canonical Male Meiosis in a Marine Gastropod, <i>Littorina saxatilis</i>.

Biology·2025
Same author

The nuclear DNA and RNA distribution in Pelomyxa spp. (Amoebozoa, Archamoebae, Pelobiontida) revealed by a simple-to-use DAPI/pyronin staining method.

The Journal of eukaryotic microbiology·2025
Same author

New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).

Protoplasma·2025
Same author

Nuclear Distribution of the Chromatin-Remodeling Protein ATRX in Mouse Early Embryos during Normal Development and Developmental Arrest In Vitro.

Life (Basel, Switzerland)·2024

Related Experiment Video

Updated: May 11, 2026

Nuclear Migration in the Drosophila Oocyte
04:17

Nuclear Migration in the Drosophila Oocyte

Published on: May 13, 2021

Nuclear structures in Tribolium castaneum oocytes.

Dmitry S Bogolyubov1, Florina M Batalova, Artyom M Kiselyov

  • 1Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russia. dmitr@mail.cytspb.rssi.ru

Cell Biology International
|May 21, 2013
PubMed
Summary

This study reveals the karyosphere in Tribolium castaneum oocytes maintains transcriptional activity and has a unique capsule involved in snRNP biogenesis. Unusual SC35 nuclear domains are linked to mRNA export, not snRNP biogenesis.

Keywords:
Cajal bodiesinterchromatin granule clusterskaryospherekaryosphere capsulenuclear bodiesoocyte nucleus

More Related Videos

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Related Experiment Videos

Last Updated: May 11, 2026

Nuclear Migration in the Drosophila Oocyte
04:17

Nuclear Migration in the Drosophila Oocyte

Published on: May 13, 2021

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Genetics

Background:

  • The karyosphere and extrachromosomal nuclear bodies are crucial for oocyte development but their precise functions and composition remain incompletely understood.
  • Previous research has not detailed the ultrastructural and immunomorphological characteristics of these nuclear structures in the red flour beetle, Tribolium castaneum.

Purpose of the Study:

  • To elucidate the ultrastructural and immunomorphological features of the karyosphere and extrachromosomal nuclear bodies in Tribolium castaneum oocytes.
  • To investigate the transcriptional activity of chromosomes within the karyosphere and identify associated proteins.
  • To characterize the composition and potential function of the karyosphere capsule and unusual SC35 nuclear domains.

Main Methods:

  • Ultrastructural analysis and immunomorphology.
  • BrUTP assay to assess transcriptional activity.
  • Detection of specific proteins and nucleic acids (RNA polymerase II, transcription factors, structural proteins, snRNAs, poly(A)+ RNAs) using immunodetection and microinjection techniques.

Main Results:

  • The karyosphere in T. castaneum oocytes is transcriptionally active until late oogenesis, with hyperphosphorylated RNA polymerase II and basal transcription factors present.
  • The karyosphere possesses an extrachromosomal capsule containing structural proteins and enriched in TMG-capped snRNAs, suggesting a role in snRNP biogenesis.
  • Unusual SC35 nuclear domains, identified as fibrillar complex bodies, contain poly(A)+ RNAs and mRNA export proteins, indicating a role in mRNP biogenesis.

Conclusions:

  • The karyosphere capsule in T. castaneum oocytes plays a structural role and is involved in snRNP biogenesis.
  • SC35 nuclear domains in T. castaneum oocytes are distinct from typical IGCs and are likely involved in mRNA export processes.
  • This study provides novel insights into the molecular organization and functional roles of nuclear bodies during insect oogenesis.