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Updated: May 11, 2026

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
Published on: February 15, 2016
Elucidating changes in interfacial water structure upon protein adsorption
1Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Abstract:
A coat to fit the body: Although protein adsorption onto surfaces is a complicated process, sum-frequency generation (SFG) spectroscopy probes the interfacial water structure around a silica surface as a model protein (bovine serum albumin, BSA) adsorbs onto it from solution. At pH 8.0, the attenuated SFG intensity after BSA adsorption indicates that interfacial water molecules are less ordered due to a reduction in surface ξ-potential. The SFG spectrum shows the C-O stretching vibrations subject to stronger ([symbol: see text]≈3200 cm(-1)) and weaker (3200 cm(-1)) hydrogen bonds.
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