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Updated: May 11, 2026
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
Published on: May 2, 2025
Comparative proteomics and correlated signaling network of kidney in ApoE deficient mouse
Xiaoyan Lv1, Jianzhong Ai, Mi Li
11Department of Dermatology, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, P.R. China; Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, P.R. China.
Purpose:
Apolipoprotein E knockout (apoE-/- ) mouse is one of the most popular models for cardiovascular research, especially in the study of atherosclerosis. Naturally, large amount of studies try to uncover the role of apoE in atherosclerosis, and indeed apoE plays an important role in this pathogenesis. Kidney is an organ that contains lots of capillaries and also largely expresses apoE. Moreover, a protective role of apoE in kidney as an autocrine regulator has been demonstrated previously, however, the underlying mechanism is largely unknown.
Experimental Design:
In this study, comparative proteomics is for the first time used to identify the differential proteins in kidneys of apoE-/- and wild type (WT) mice, respectively, and we try to reveal the signaling network of apoE in mice kidney using bioinformatics analysis.
Results:
Our findings show that approximately 80 proteins are significantly differentially expressed in kidneys of apoE-/- and WT mice, and the signaling network correlated to apoE is successfully established by employing bioinformatics assay.
Conclusions And Clinical Relevance:
Taken together, we originally identify the proteins with differential expression and propose an apoE correlated molecular network in mice kidney. These findings further provide evidence of the role of apoE in mice kidney and a brand new perspective in the protection and treatment of kidney disease.
Insights
Apolipoprotein E (apoE) knockout mice kidneys show differential protein expression compared to wild-type. This study establishes an apoE-correlated molecular network, offering new insights into kidney disease mechanisms and treatment.
Area of Science:
- Proteomics
- Bioinformatics
- Cardiovascular Research
Background:
- Apolipoprotein E (apoE) knockout mice are crucial models for atherosclerosis research.
- ApoE plays a protective role in the kidney, but its mechanism is not fully understood.
Purpose of the Study:
- To identify differential proteins in the kidneys of apoE knockout and wild-type mice using comparative proteomics.
- To elucidate the signaling network of apoE in the mouse kidney through bioinformatics analysis.
Main Methods:
- Comparative proteomics was employed to analyze kidney tissue from apoE knockout and wild-type mice.
- Bioinformatics analysis was used to establish the apoE-correlated signaling network.
Main Results:
- Approximately 80 proteins exhibited significant differential expression in the kidneys of apoE knockout versus wild-type mice.
- A signaling network correlated with apoE function in the kidney was successfully established.
Conclusions:
- This study uniquely identifies differentially expressed proteins and proposes an apoE-correlated molecular network in mouse kidneys.
- Findings provide evidence for apoE's role in the kidney and suggest new perspectives for kidney disease protection and treatment.
