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Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Laboratory evaluation of a rapid diagnostic test for Neisseria meningitidis serogroup A

Aude Terrade1, Jean-Marc Collard, Farida Nato

  • 1Institut Pasteur, Invasive Bacterial Infection Unit, National Reference Centre for Meningococci, and WHO Collaborating Centre for Meningococci, Paris, France.

Transactions of the Royal Society of Tropical Medicine and Hygiene
|May 21, 2013
PubMed
Abstract

Insights

A new rapid detection test (RDT) effectively identifies serogroup A in cerebrospinal fluid samples, aiding surveillance for meningococcal meningitis outbreaks in Africa.

Area of Science:

  • Microbiology
  • Epidemiology
  • Public Health

Background:

  • Recurrent epidemics of meningococcal meningitis occur in Africa's meningitis belt.
  • Serogroup A is a primary, but not exclusive, cause of these outbreaks.

Purpose of the Study:

  • To evaluate a novel rapid detection test (RDT) for Neisseria meningitidis serogroup A.

Main Methods:

  • The RDT was tested using 401 cerebrospinal fluid (CSF) samples.
  • Performance was assessed against established diagnostic criteria.

Main Results:

  • The RDT demonstrated a detection limit of 10(5) CFU/ml.
  • Sensitivity for serogroup A detection in CSF was 88%, with a specificity of 99%.

Conclusions:

  • The developed RDT is suitable for field surveillance of meningococcal meningitis.
  • This tool can assist in characterizing meningitis cases, especially post-introduction of the serogroup A conjugate vaccine.
Keywords:
DiagnosisMeningitisNeisseria meningitidisRapid testSerogroup A

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