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Related Experiment Video

Updated: May 11, 2026

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
07:53

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

Published on: April 1, 2016

A 3D printed fluidic device that enables integrated features.

Kari B Anderson1, Sarah Y Lockwood, R Scott Martin

  • 1Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.

Analytical Chemistry
|May 22, 2013
PubMed
Summary
This summary is machine-generated.

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A novel 3D printed fluidic device enables drug transport studies. This reusable device allows antibiotic migration through membranes and demonstrates cellular response to reagents, aiding drug development.

Area of Science:

  • Biotechnology
  • Microfluidics
  • 3D Printing

Background:

  • Conventional soft lithography is a common prototyping method for fluidic devices.
  • Three-dimensional (3D) printing offers a one-step production approach for complex devices.
  • Integrating fluidic channels with cell culture membranes is crucial for studying drug transport and cellular effects.

Purpose of the Study:

  • To develop a reusable, high-throughput 3D printed fluidic device for drug transport and cell-based assays.
  • To enable molecular transport from microchannels to cell culture surfaces.
  • To validate the device's utility in studying antibiotic migration and cellular responses.

Main Methods:

  • Fabrication of a multi-channel fluidic device using 3D printing technology.

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Last Updated: May 11, 2026

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
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Published on: April 1, 2016

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  • Integration of the device with commercially available porous membrane inserts.
  • Perfusion of antibiotic solutions (levofloxacin, linezolid) through the channels.
  • Assessment of drug migration across the membrane via quantitative analysis.
  • Application of saponin to cell cultures on the membrane to evaluate cellular response using fluorescence.

    • Main Results:

    • The 3D printed fluidic device successfully facilitated controlled flow and molecular transport.
    • Approximately 18-21% of pumped antibiotics migrated through the porous membrane.
    • Mammalian cells cultured on the membrane showed a 4-fold increase in fluorescence upon treatment with saponin, indicating compromised cell membranes.

    Conclusions:

    • The developed 3D printed fluidic device is a viable tool for studying drug transport and its effects on cells.
    • The device's design allows for high-throughput screening and integration with standard cell culture techniques.
    • This technology holds promise for advancing drug discovery and personalized medicine research.