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Levansucrase optimization using solid state fermentation and levan biological activities studies.

Mona A Esawy1, Azza M Abdel-Fattah, Mamdouh M Ali

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Carbohydrate Polymers
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Summary
This summary is machine-generated.

Bacillus subtilis produced levansucrase, an enzyme with significant antitumor and fibrinolytic activities. Optimization using statistical designs enhanced enzyme productivity for potential therapeutic applications.

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Area of Science:

  • Biotechnology
  • Enzymology
  • Microbiology

Background:

  • Levansucrase from Bacillus subtilis is a valuable enzyme with potential industrial and therapeutic applications.
  • Solid-state fermentation offers an efficient method for enzyme production.
  • Optimization of fermentation conditions is crucial for maximizing enzyme yield.

Purpose of the Study:

  • To optimize the solid-state fermentation of Bacillus subtilis NRC1aza for enhanced levansucrase production.
  • To investigate the antitumor and fibrinolytic activities of the produced levansucrase variants (LevCR and LevQT).

Main Methods:

  • Solid-state fermentation using starch as a support for Bacillus subtilis NRC1aza.
  • Sequential optimization strategy employing Plackett-Burman and fractional factorial designs.
  • In vitro assays to evaluate antitumor activity against cancer cell lines (Ehrlich ascites carcinoma, MCF-7, HepG2) and fibrinolytic activity.

Main Results:

  • Maximal levansucrase productivity of 170 U/gds was achieved under optimized conditions (glucose, yeast extract, pH 8).
  • LevCR and LevQT demonstrated significant antitumor activity against various human and animal cancer cell lines.
  • LevQT showed comparable efficacy to cisplatin against cancer cells.
  • LevCR was identified as a potent fibrinolytic agent.

Conclusions:

  • The study successfully optimized levansucrase production using statistical experimental designs.
  • Levansucrase variants LevCR and LevQT exhibit promising antitumor and fibrinolytic properties, respectively.
  • These findings highlight the therapeutic potential of Bacillus subtilis-derived levansucrase for cancer treatment and thrombosis management.