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Related Concept Videos

Biofuels01:25

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
Green Algae01:21

Green Algae

Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
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Bioremediation is an environmentally sustainable process that employs living organisms—primarily microorganisms—to degrade or neutralize pollutants from contaminated environments. In oil spills and hydrocarbon pollution, bioremediation involves the use of hydrocarbon-degrading bacteria to transform toxic compounds into less harmful substances. This approach leverages natural microbial metabolic processes and is considered both cost-effective and ecologically favorable compared to physical or...
Lipid Catabolism01:25

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Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Bioremediation00:46

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

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Related Experiment Video

Updated: May 11, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

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Published on: January 7, 2019

Microalgae harvesting and subsequent biodiesel conversion.

Dang-Thuan Tran1, Bich-Hanh Le, Duu-Jong Lee

  • 1Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

Bioresource Technology
|May 22, 2013
PubMed
Summary
This summary is machine-generated.

Immobilized lipase efficiently converts microalgae oil from chitosan-coagulated Chlorella vulgaris into biodiesel, achieving high yields comparable to centrifugation methods. This enzymatic approach offers a sustainable alternative for biofuel production.

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Published on: June 24, 2016

Area of Science:

  • Biotechnology
  • Renewable Energy
  • Chemical Engineering

Background:

  • Microalgae, such as Chlorella vulgaris ESP-31, are a promising source of lipids for biodiesel production.
  • Efficient harvesting and dewatering of microalgae biomass are critical steps for cost-effective biodiesel synthesis.
  • Coagulation and centrifugation are common methods for microalgae harvesting, but their impact on subsequent transesterification needs evaluation.

Purpose of the Study:

  • To evaluate the effectiveness of chitosan and polyaluminium chloride (PACl) for harvesting Chlorella vulgaris ESP-31.
  • To compare the biodiesel conversion efficiency using microalgae harvested by coagulation and centrifugation.
  • To assess the performance of immobilized Burkholderia lipase and a synthesized solid catalyst (SrO/SiO2) for transesterification.

Main Methods:

  • Chlorella vulgaris ESP-31 was harvested using chitosan and PACl coagulation, and centrifugation.
  • Biodiesel production was carried out via transesterification using immobilized Burkholderia lipase and SrO/SiO2 solid catalyst.
  • The impact of coagulants (PACl) and water content on transesterification efficiency was investigated.

Main Results:

  • Immobilized lipase achieved a high biodiesel conversion of 97.6% with wet, chitosan-coagulated microalgae, comparable to centrifugation (97.1%).
  • PACl significantly inhibited both enzymatic and chemical transesterification processes.
  • The solid catalyst SrO/SiO2 showed good performance (80% conversion) with centrifuged, water-removed microalgae but lower conversion with coagulated biomass (55.7-61.4%).
  • The immobilized lipase retained activity over three reuse cycles.

Conclusions:

  • Immobilized lipase is a robust catalyst for biodiesel production from chitosan-coagulated microalgae, even with residual moisture.
  • Chitosan coagulation followed by enzymatic transesterification presents a viable and efficient route for microalgae-based biodiesel.
  • PACl is unsuitable as a coagulant for microalgae intended for biodiesel production due to transesterification inhibition.