Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sample Preparation for Analysis: Overview

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhanced Mechanical Properties Via Boron-Induced Multi-Mechanism Strengthening in (NbTaTi<sub>1.5</sub>V)<sub>100-x</sub>B<sub>x</sub> Refractory High Entropy Alloys.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Nanoscale Coherent Phonons with Broadband Frequency Tunability.

Physical review letters·2026

Same author

Interpretable fine-tuned large language models facilitate making genetic test decisions for rare diseases.

NPJ digital medicine·2026

Same author

Toxicity Test of Total Particulate Matter in Mainstream Cigarette Smoke Based on Lung-Hepatocyte Vascularised Microfluidic Chip.

Basic & clinical pharmacology & toxicology·2026

Same author

Interpretable Fine-tuned Large Language Models Facilitate Making Genetic Test Decisions for Rare Diseases.

medRxiv : the preprint server for health sciences·2026

Same author

Integrating Chain-of-Thought and Retrieval Augmented Generation Enhances Rare Disease Diagnosis from Clinical Notes.

ArXiv·2026

Search research articles

Related Experiment Video

Updated: May 11, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Development of solvent micro-extraction combined with derivatization.

Huaqing Lin¹, Jiale Wang, Lingjie Zeng

¹Technical Center, Shanghai Tobacco Group Co., Ltd, 200082 Shanghai, China.

Journal of Chromatography. A

|May 22, 2013

Summary

Solvent microextraction (SME) techniques like SDME, HF-LPME, and DLLME are advanced by coupling them with derivatization. This combination enhances analytical capabilities for a wide range of compounds.

More Related Videos

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 11, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

Analytical Chemistry
Separation Science

Background:

Solvent microextraction (SME) encompasses techniques like single-drop microextraction (SDME), hollow-fiber liquid-phase microextraction (HF-LPME), and dispersive liquid-liquid microextraction (DLLME).
These methods offer broad applicability for analyte extraction.

Purpose of the Study:

To review and highlight advancements in microextraction technologies, specifically SME techniques.
To emphasize the strategic integration of microextraction with derivatization in analytical chemistry.
To discuss the coupling of microextraction-derivatization with various analytical instruments.

Main Methods:

Review of single-drop microextraction (SDME).
Review of hollow-fiber liquid-phase microextraction (HF-LPME).
Review of dispersive liquid-liquid microextraction (DLLME).
Discussion of microextraction coupled with derivatization strategies.
Analysis of coupling with GC, GC-MS, and HPLC.

Main Results:

Microextraction techniques show significant potential for diverse analyte extraction.
Combining microextraction with derivatization substantially improves separation, detectability, and compound identification.
The integration expands the scope and enhances the effectiveness of analytical methods.

Conclusions:

Microextraction coupled with derivatization offers a powerful approach in analytical chemistry.
Further development in microextraction-derivatization is encouraged.
Successful coupling with techniques like GC-MS and HPLC broadens analytical applications.