Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Cystic Fibrosis: Management01:24

Cystic Fibrosis: Management

434
Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...
434
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

674
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
674
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.3K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.5K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.5K
Antiasthma Drugs: Leukotriene Modifiers01:19

Antiasthma Drugs: Leukotriene Modifiers

1.7K
Leukotriene modifiers, or cysteinyl leukotriene receptor antagonists, are medications used to manage chronic asthma. These agents target specific inflammatory mediators produced during arachidonic acid metabolism, an essential process in generating inflammation in the body.
Leukotriene modifiers work through two distinct mechanisms:
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibroblast-specific deletion of Yap/Taz impairs mouse postnatal dermal development by diminishing collagenous matrix.

The Journal of investigative dermatology·2026
Same author

4PL-Driven optimization of electric vehicle battery recycling networks: enhancing greenness, timeliness and resilience against disruptions.

Waste management (New York, N.Y.)·2026
Same author

Robotic transvaginal hybrid natural orifice transluminal endoscopic surgery (NOTES) nephrectomy: clinical experience with 23 cases.

Surgical endoscopy·2026
Same author

Analysis of the predictive value of heart rate variability analysis combined with SOFA and APACHE II scores for the 28-day mortality risk of patients in the emergency intensive care unit.

Frontiers in medicine·2026
Same author

Nowcasting of harmful algal blooms based on preceding environmental dynamics in coastal areas of the Beibu Gulf.

Water research·2026
Same author

Research updates in cystic fibrosis related diabetes: Understanding pathophysiology, expanding animal and human islet models, and advancing clinical and translational research.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2026

Related Experiment Videos

Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer.

Zhaolin Zhang1, Shih-Hsing Leir, Ann Harris

  • 1Human Molecular Genetics Program, Lurie Children's Research Center, and Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.

Molecular and Cellular Biology
|May 22, 2013
PubMed
Summary

A specific DNA region regulates cystic fibrosis transmembrane conductance regulator (CFTR) gene expression in airways. This enhancer element involves key proteins like IRF1 and NF-Y, impacting CFTR levels through distinct mechanisms.

Related Experiment Videos

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • The cystic fibrosis transmembrane conductance regulator (CFTR) gene is crucial for lung function.
  • An airway-selective DNase-hypersensitive site (DHS-35kb) upstream of the CFTR gene exhibits enhancer activity in lung epithelial cells.
  • This region is enriched for the epigenetic mark H3K4me1, suggesting a role in active gene regulation.

Purpose of the Study:

  • To identify and characterize the functional enhancer element within DHS-35kb.
  • To elucidate the molecular mechanisms by which DHS-35kb regulates CFTR expression in airway cells.
  • To investigate the roles of specific transcription factors and epigenetic modifiers in this regulatory process.

Main Methods:

  • Deletion analysis to define the core enhancer region.
  • Electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) to assess protein binding (IRF1, NF-Y).
  • siRNA-mediated gene silencing and overexpression studies to evaluate the functional impact of regulatory proteins (IRF1, IRF2, NF-YA, SETD7).

Main Results:

  • A 350-bp region within DHS-35kb possesses full enhancer activity and binds IRF1 and NF-Y.
  • IRF1 depletion or IRF2 overexpression reduces CFTR expression.
  • NF-Y is essential for maintaining H3K4me1 enrichment at DHS-35kb, and SETD7-mediated H3K4 monomethylation is required for NF-Y binding.

Conclusions:

  • The DHS-35kb element is a critical airway-selective enhancer for CFTR gene regulation.
  • CFTR expression is modulated by IRF1 and NF-Y through distinct pathways.
  • Epigenetic modifications, specifically H3K4me1, play a role in facilitating transcription factor binding and enhancer function.