Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A computerized method for optimization of radioimmunoassays].

F Rousseau1, J C Forest

  • 1Service de Biochimie et Centre de Recherche de l'Hôpital Saint-François-d'Assise, Québec, Canada.

Annales De Biologie Clinique
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How to compensate for frailty? The real life impact of geriatric co-management on morbi-mortality after colorectal cancer surgery in patients aged 70 years or older.

Aging clinical and experimental research·2024
Same author

Comprehensive analysis of synthetic learning applied to neonatal brain MRI segmentation.

Human brain mapping·2024
Same author

The effect of mutation on an aggregation-prone protein: An in vivo, in vitro, and in silico analysis.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Low prevalence of tissue detection of cefepime and daptomycin used as empirical treatment during revision for periprosthetic joint infections: results of a prospective multicenter study.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2021
Same author

Introducing cell-free DNA noninvasive testing in a Down syndrome public health screening program: a budget impact analysis.

Cost effectiveness and resource allocation : C/E·2020
Same author

Global Perturbation of Initial Geometry in a Biomechanical Model of Cortical Morphogenesis.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020

This study introduces a rapid, one-step method for optimizing radioimmunoassays (RIA). The technique uses saturation curves and a Scatchard plot to predict standard curve characteristics, significantly improving RIA development.

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Context:

  • Radioimmunoassays (RIA) are widely used for quantifying substances.
  • Optimizing RIA protocols can be time-consuming and complex.
  • Accurate optimization is crucial for reliable assay performance.

Purpose:

  • To develop a rapid, one-step quantitative method for radioimmunoassay optimization.
  • To create a predictive tool for standard curve characteristics and assay sensitivity.
  • To streamline and enhance the RIA development process.

Summary:

  • A novel method utilizes saturation curves and Scatchard plot analysis to determine optimal antiserum titres.
  • A predictive table and microcomputer program are generated to forecast standard curve parameters (Bo/T, Bo, T) and assay sensitivity.

Related Experiment Videos

  • Experimental validation with 17-beta-estradiol and hLH assays confirmed the accuracy of the predictive method.

    • Impact:

    • Accelerates the optimization of radioimmunoassays, saving time and resources.
    • Improves the overall process of RIA development and validation.
    • Enhances the reliability and sensitivity of RIA results for various applications.