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Related Experiment Video

Updated: May 11, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Angiosarcoma: a tissue microarray study with diagnostic implications.

Priya Rao1, Guy Lahat, Christina Arnold

  • 1Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030-4009, USA. prao@mdanderson.org

The American Journal of Dermatopathology
|May 22, 2013
PubMed
Summary

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Angiosarcoma (AS) often shows unusual patterns of endothelial markers, with most tumors expressing multiple markers. These patterns may indicate important biological characteristics but did not clearly correlate with disease-specific survival in this study.

Area of Science:

  • Oncology
  • Pathology
  • Biomarker Research

Background:

  • Angiosarcoma (AS) is a rare soft tissue sarcoma characterized by endothelial differentiation.
  • Immunohistochemical markers like CD31, D2-40, factor VIII, and CD34 are used to identify endothelial differentiation in AS.
  • Understanding the expression patterns of these markers is crucial for diagnosis and potentially for understanding AS biology.

Purpose of the Study:

  • To investigate the expression patterns of immunohistochemical markers of endothelial differentiation in angiosarcoma.
  • To correlate these marker patterns with clinicopathologic features and patient outcomes, specifically disease-specific survival (DSS).

Main Methods:

  • Construction of an angiosarcoma tissue microarray from 70 specimens.
  • Immunohistochemical analysis of the tissue microarray for CD31, CD34, factor VIII, D2-40, and pan-cytokeratin.

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  • Linking microarray data to patient clinicopathologic and outcome data, followed by univariate analyses for DSS factors.

    • Main Results:

    • 92% of angiosarcomas expressed at least one endothelial marker, with 88% expressing two or more.
    • Factor VIII (83%), CD31 (80%), CD34 (63%), and D2-40 (43%) were the most common markers.
    • Unusual coexpression of D2-40 and CD31 was observed in 88% of D2-40 positive tumors.
    • No single endothelial marker showed a clear association with disease-specific survival.
    • Epithelioid angiosarcomas showed a higher rate of keratin expression (50%) compared to non-epithelioid types (9%).

    Conclusions:

    • Angiosarcoma frequently exhibits aberrant expression patterns and loss of endothelial markers.
    • The use of multiple immunohistochemical markers is recommended for diagnosing challenging angiosarcoma cases.
    • Observed marker patterns may reflect underlying biological characteristics of angiosarcoma.