Enhanced transesterification of ethyl ferulate with glycerol for preparing glyceryl diferulate using a lipase in
Shangde Sun1, Fei Qin, Yanlan Bi
1Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, Lianhua Road, Zhengzhou, 450001, Henan, People's Republic of China. sunshangde@hotmail.com
Abstract:
Glyceryl diferulate (DFG) is a water-soluble ester of ferulic acid. A novel ionic liquid (IL) system for enzymatic transesterification of ethyl ferulate (EF) with glycerol to produce DFG was developed. Of three ILs with different anions (BF4 (-), PF6 (-) and TF2N(-)) and cations (BDMIM, OMIM, HMIM, BMIM, and EMIM), EMIMTF2N proved the best using a commercial lipase. It had a significant protective effect against thermal inactivation of the enzyme. High EF conversion (~100 %) and DFG yield (45 %) were achieved using 45 mg enzyme/ml; temperature, 70 °C; reaction time, 12 h.
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