Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

EMito-Metrix enables automated evaluation of mitochondrial morphology across species.

Nature metabolism·2025

Same author

Combined tendon reflex and motor evoked potential recordings in amyotrophic lateral sclerosis.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2023

Same author

Epigenetically regulated PCDHB15 impairs aggressiveness of metastatic melanoma cells.

Clinical epigenetics·2022

Same author

A Spectral Principal Component Analysis-Based Framework for Composite Hard/Soft Tissue Fluorescence Image Investigation.

Frontiers in physiology·2022

Same author

Single-cell imaging of the cell cycle reveals CDC25B-induced heterogeneity of G1 phase length in neural progenitor cells.

Development (Cambridge, England)·2022

Same author

Quantitative Analysis of Cell Aggregation Dynamics Identifies HDAC Inhibitors as Potential Regulators of Cancer Cell Clustering.

Cancers·2021

Search research articles

Related Experiment Video

Updated: May 11, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

A versatile sample holder for single plane illumination microscopy.

Annaïck Desmaison¹, Corinne Lorenzo, Jacques Rouquette

¹Université de Toulouse, ITAV-UMS3039, F-31106, Toulouse, France.

Journal of Microscopy

|May 23, 2013

Summary

This summary is machine-generated.

A novel 3D-printed hydrogel sample holder enhances live imaging with Single Plane Illumination Microscopy (SPIM). This innovative approach avoids limitations of traditional agarose embedding, supporting cell division and enabling long-term, whole-organism studies.

Keywords:

Light sheet microscopy SPIM live imaging multicellular tumour spheroid sample holder single/selective plane illumination microscopy

More Related Videos

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

Related Experiment Videos

Last Updated: May 11, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

Area of Science:

Biomedical Engineering
Microscopy Technology
Cell Biology

Background:

Single Plane Illumination Microscopy (SPIM) is valuable for live imaging of entire organisms.
Traditional sample preparation using agarose or gel embedding poses limitations for time-lapse studies.
Developing improved sample handling is crucial for advancing SPIM applications.

Purpose of the Study:

To develop an innovative sample holder for SPIM that overcomes limitations of current embedding methods.
To create a versatile and easily producible sample container system for live cell imaging.
To assess the impact of the new holder on cell viability and division during time-lapse experiments.

Main Methods:

A novel holder device was designed and produced using 3D printing for creating hydrogel sample containers.
The holder system was tested for its efficacy in preparing samples for SPIM.
Time-lapse recordings of multicellular tumor spheroid growth were performed using the new holder.

Main Results:

The 3D-printed holder enables the straightforward preparation of hydrogel-based sample containers.
Multicellular tumor spheroids were successfully imaged over time using the new SPIM sample holder.
Crucially, cell division was not impaired with the hydrogel holder, unlike with traditional gel embedding.

Conclusions:

The developed 3D-printed hydrogel sample holder offers a versatile and effective solution for SPIM live imaging.
This method overcomes key limitations associated with agarose/gel embedding, particularly for time-lapse experiments.
This customizable sample holder concept has the potential to significantly promote the wider adoption of SPIM technology.