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Published on: January 12, 2015
Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells
1State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro.
Materials And Methods:
Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level.
Results:
Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein.
Conclusions:
In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.
