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Effects of leptin on fracture healing in rat tibia
Gökçen Kerimoğlu1, Esin Yuluğ, Servet Kerimoğlu
1Department of Histology and Embryology, Kanuni Training and Research Hospital, Trabzon, Turkey.
Objectives:
In this study, we aimed to evaluate possible effects of leptin on fracture healing.
Materials And Methods:
Between November 2009 and May 2010, 54 male Sprague Dawley rats with a mean age of 16 weeks and a mean body weight of 323 g (range; 295-352 g) were included and randomly divided into three equal groups. Standardized tibial fractures were created to all rats. Group 1 was treated with 0.5 mL physiological saline, group 2 was treated with 0.1 µg/kg leptin within 0.5 mL physiological saline, and group 3 was treated 0.3 µg/kg leptin within 0.5 mL physiological saline for 14 days intraperitoneally. Each group was divided into three subgroups including six rats for the evaluation at second, third and fifth weeks.
Results:
Radiological evaluation showed that fracture healing of group 3 was better than group 1 (p=0.014) only at fifth week. Histological evaluation revealed fracture healing of group 3 was better than group 1 at third week (p=0.011). At fifth week, both group 2 and group 3 had better fracture healing than group 1 (p=0.006, p=0.002, respectively). There was no statistically significant difference in fracture healing between group 2 and 3 at fifth week (p=0.176).
Conclusion:
Leptin has a positive dose-dependent effect on rat tibial fracture healing.
