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Related Concept Videos

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Related Experiment Video

Updated: May 11, 2026

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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iFASP: combining isobaric mass tagging with filter-aided sample preparation.

Gary S McDowell1, Aleksandr Gaun, Hanno Steen

  • 1Proteomics Center and Department of Pathology, Boston Children's Hospital, Boston, Massachusetts 02115, USA.

Journal of Proteome Research
|May 23, 2013
PubMed
Summary
This summary is machine-generated.

We developed iFASP, a new protocol for mass spectrometry proteomics. This method improves sample preparation for quantitative proteomics, doubling peptide identifications and improving data reliability.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biochemistry

Background:

  • Reproducible mass spectrometry proteomics requires meticulous sample preparation.
  • Quantitative proteomics using chemical isobaric labeling (tandem mass tagging) is sensitive to sample processing steps.
  • Combining labeled samples late in processing necessitates robust upstream methods.

Purpose of the Study:

  • To introduce iFASP, a protocol integrating filter-aided sample preparation (FASP) with isobaric mass tagging.
  • To provide a simple, effective method for clean sample preparation, efficient digestion, and high labeling yields.
  • To evaluate iFASP's performance in complex biological samples.

Main Methods:

  • Integration of the filter-aided sample preparation (FASP) method with chemical isobaric labeling (tandem mass tagging).
  • Application of the iFASP protocol to complex Xenopus laevis egg and embryo lysates.
  • Comparison of iFASP with standard in-solution digestion and labeling protocols.

Main Results:

  • iFASP provides a quick, simple, and effective method for sample preparation.
  • Labeling efficiency for both iFASP and standard methods exceeds 99%.
  • iFASP doubled the number of high-confidence peptide identifications and quantified peptide spectral matches compared to the standard method.

Conclusions:

  • iFASP is a robust and reliable sample preparation protocol for quantitative proteomics.
  • The iFASP method significantly enhances peptide identification and quantification in complex samples.
  • This protocol improves data quality and reproducibility in mass spectrometry-based proteomics.