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Decontamination of materials contaminated with Bacillus anthracis and Bacillus thuringiensis Al Hakam spores using
T L Buhr1, C M Wells, A A Young
1CBR Concepts and Experimentation Branch (Z21), Naval Surface Warfare Center, Dahlgren Division, Dahlgren, VA 22448-5162, USA. DLGR_NSWC_Z20@navy.mil
Aims:
To develop test methods and evaluate survival of Bacillus anthracis Ames, B. anthracis ∆Sterne and B. thuringiensis Al Hakam spores after exposure to PES-Solid (a solid source of peracetic acid), including PES-Solid formulations with bacteriostatic surfactants.
Methods And Results:
Spores (≥ 7 logs) were dried on seven different test materials and treated with three different PES-Solid formulations (or preneutralized controls) at room temperature for 15 min. There was either no spore survival or less than 1 log (<10 spores) of spore survival in 56 of 63 test combinations (strain, formulation and substrate). Less than 2.7 logs (<180 spores) survived in the remaining seven test combinations. The highest spore survival rates were seen on water-dispersible chemical agent resistant coating (CARC-W) and Naval ship topcoat (NTC). Electron microscopy and Coulter analysis showed that all spore structures were intact after spore inactivation with PES-Solid.
Conclusions:
Three PES-Solid formulations inactivated Bacillus spores that were dried on seven different materials.
Significance And Impact Of The Study:
A test method was developed to show that PES-Solid formulations effectively inactivate Bacillus spores on different materials.
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